Description of apparatus:
The lambda 25 are versatile spectrometers operating in the ultraviolet (UV) and visible (Vis) spectral ranges. The spectrometers feature a double-beam, all reflecting system.
Lambda 25 spectrometers are usable in a wide range of applications as indicated by their performance specification.
Applications for UV-VIS spectroscopy include quantification of nucleic acids, proteins, and cells, as well as monitoring enzyme reactions, along with a variety of other uses. Even in the traditional application areas, such as materials characterization, optics, coatings, glass, pharmaceutical QA/QC, and color control, UV/VIS spectroscopy has become more sophisticated, providing higher quality results faster and easier than ever. The Lambda 25 UV/VIS Spectrophotometer, a product of PerkinElmer Inc., is a solid and robust system fit for these needs. The system is easy to operate and delivers results the user can trust with minimal operator training. The key feature is it’s easy to develop, simple, robust methods; this ensures that the methods are followed without mistakes. This is an ideal match for most routine UV/VIS applications, including regulatory tests, giving fast and reliable results time after time.
Experiments done on it:
- This unit used for research.
- Mixing Behavior under Various Operating Conditions
The objective of this experiment is to study the behavior of mixing under various operating.
Mixing is applied to the processes used to reduce the degree of non-uniformity or gradient of a property in a system such as concentration, viscosity, temperature and so on.
There are many types of mixing: Single Phase Liquid Mixing, Mixing of Immiscible Liquids, Gas- Liquid Mixing, Liquid-Solid Mixing and Gas-Liquid-Solid Mixing
Mixing time is the time required producing a mixture or a product of predetermined quality, and the rate of mixing is the rate at which the mixing progresses towards the final state.
For a single-phase liquid in a stirred tank to which a volume of tracer materials is added, the mixing time is measured from the instant the tracer is added to the time when the contents of the vessel have reached the required degree contents of uniformity.
The mixing time will depend upon the process and the following: System geometry, Impeller Diameter (D), Speed of rotation (revs/unit time) N, Properties of liquids. t = f (N, D, g, ρ, μ, system geometry)
- Scale up of stirred tank reactor
The objective of this experiment is to apply different scale up rules on scaling 200ml batch to 2000ml.
In this experiment the molecular spectroscopy (LAMBDA 25) is used to find the concentration of the solution.